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cd44 apc  (R&D Systems)


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    R&D Systems cd44 apc
    Cd44 Apc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd44+apc/Human+CD44+v6+PE-conjugated+Antibody/pm41631682-53-21-27
    Average 93 stars, based on 16 article reviews
    cd44 apc - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Genetic attenuation of ALDH1A1 increases metastatic potential and aggressiveness in colorectal cancer.
    Article Snippet: .. Primary antibodies in concentrations according to the manufacturer’s instructions were added: CD326-PE (EpCAM, #130-093-440, Miltenyi Biotec), CD324-APC (E-cadherin, #130-111-993, Miltenyi Biotec), CD44-APC (#130-113-331, Miltenyi Biotec), CD44v6-PE (FAB3660P, R&D Systems, Abingdon, UK), cMET-APC (FAB3582A, R&D Systems), CD325-APC (N-cadherin, #130-116-274, Miltenyi Biotec), CD87-APC (#130-114-893, Miltenyi Biotec), CD338-PE (ABCG2, #12888841, eBiosciences, Altrincham, UK); and incubated for 15 min in the dark. ..

    Article Title: A Novel Animal Model for Locally Advanced Breast Cancer
    Article Snippet: .. Flow cytometry IOWA-1T cells were detached by trypsin, washed by PBS and incubated with CD24 – PE (eBioscience) and CD44 – APC (RD Systems) antibodies on ice for 30 min. Hoechst Stains (Invitrogen) was used for living cells separation. .. Flow cytometry was performed on a FACS (fluorescence-activated cell sorting) DiVa (Becton Dickinson, Franklin Lakes, NJ, USA) machine.

    Article Title: A Novel Animal Model for Locally Advanced Breast Cancer
    Article Snippet: .. IOWA-1T cells were detached by trypsin, washed by PBS and incubated with CD24 – PE (eBioscience) and CD44 – APC (RD Systems) antibodies on ice for 30 min. Hoechst Stains (Invitrogen) was used for living cells separation. .. Flow cytometry was performed on a FACS (fluorescence-activated cell sorting) DiVa (Becton Dickinson, Franklin Lakes, NJ, USA) machine.

    Flow Cytometry:

    Article Title: A Novel Animal Model for Locally Advanced Breast Cancer
    Article Snippet: .. Flow cytometry IOWA-1T cells were detached by trypsin, washed by PBS and incubated with CD24 – PE (eBioscience) and CD44 – APC (RD Systems) antibodies on ice for 30 min. Hoechst Stains (Invitrogen) was used for living cells separation. .. Flow cytometry was performed on a FACS (fluorescence-activated cell sorting) DiVa (Becton Dickinson, Franklin Lakes, NJ, USA) machine.



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    Image Search Results


    Val-ILs and αPD-1 affect the growth and spread of breast tumor cells 10 6 4T1 cells were transplanted through i.d. injection into the mammary gland of female BALB/c mice. When tumors reached 50 mm 3 , mice were i.v. injected with Val-ILs (10 12 NPs) and i.p. injected with αPD-1 (200 μg) on days 6, 9, and 12. Analyses were performed on day 15 (A–E). (A) Tumor growth volumes measured in vivo. (B) Image of tumors isolated ex vivo from the mammary gland of mice (two independent in vivo experiments). Val-ILs-Combo was efficient in reducing the tumor volumes, and the combination of Val-ILs-Combo and αPD-1 was even more efficient. Mice without breast tumors are identified with a black cross. (C) FC on TAM cells in the TME and macrophages in the spleen, to identify M1-like and M2-like phenotypes. (D) Images of mice measured with in vivo imaging system technology showing the detection of bioluminescent 4T1 cells in the mammary gland, following treatment with Val-ILs-Combo and/or αPD-1. (E) FC was used to detect metastatic CD44 + GFP + 4T1 cells spreading to distant organs following treatment. Examples of FC plots and a heatmap displaying the mean percentages. Undetected (ud) indicates the absence of detectable tumor 4T1 cells. (F) On day 30, tumor-free mice were rechallenged with a secondary injection of 10 6 4T1 cells into the contralateral mammary gland. Tumor growth was monitored to evaluate the establishment of a protective anti-tumor memory. The number of mice used per group is indicated on the figure, data are shown as means ± SD, and p values are compared to Val-ILs-IgG + αIgG or between the different groups and are calculated using one-way ANOVA with Tukey’s multiple comparisons test. See also .

    Journal: Cell Reports Medicine

    Article Title: Valrubicin-loaded immunoliposomes targeting antigens on immunosuppressive cells to circumvent resistance to cancer immunotherapy

    doi: 10.1016/j.xcrm.2026.102632

    Figure Lengend Snippet: Val-ILs and αPD-1 affect the growth and spread of breast tumor cells 10 6 4T1 cells were transplanted through i.d. injection into the mammary gland of female BALB/c mice. When tumors reached 50 mm 3 , mice were i.v. injected with Val-ILs (10 12 NPs) and i.p. injected with αPD-1 (200 μg) on days 6, 9, and 12. Analyses were performed on day 15 (A–E). (A) Tumor growth volumes measured in vivo. (B) Image of tumors isolated ex vivo from the mammary gland of mice (two independent in vivo experiments). Val-ILs-Combo was efficient in reducing the tumor volumes, and the combination of Val-ILs-Combo and αPD-1 was even more efficient. Mice without breast tumors are identified with a black cross. (C) FC on TAM cells in the TME and macrophages in the spleen, to identify M1-like and M2-like phenotypes. (D) Images of mice measured with in vivo imaging system technology showing the detection of bioluminescent 4T1 cells in the mammary gland, following treatment with Val-ILs-Combo and/or αPD-1. (E) FC was used to detect metastatic CD44 + GFP + 4T1 cells spreading to distant organs following treatment. Examples of FC plots and a heatmap displaying the mean percentages. Undetected (ud) indicates the absence of detectable tumor 4T1 cells. (F) On day 30, tumor-free mice were rechallenged with a secondary injection of 10 6 4T1 cells into the contralateral mammary gland. Tumor growth was monitored to evaluate the establishment of a protective anti-tumor memory. The number of mice used per group is indicated on the figure, data are shown as means ± SD, and p values are compared to Val-ILs-IgG + αIgG or between the different groups and are calculated using one-way ANOVA with Tukey’s multiple comparisons test. See also .

    Article Snippet: APC anti-Mouse CD44 (Clone REA664) , Miltenyi Biotec , Cat# 130-119-121; RRID: AB_2751628.

    Techniques: Injection, In Vivo, Isolation, Ex Vivo, In Vivo Imaging